Open Access Research

Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications

Murali Krishna Mamidi12, Gurbind Singh1, Juani Mazmin Husin1, Kavitha Ganesan Nathan1, Gopinath Sasidharan1, Zubaidah Zakaria3, Ramesh Bhonde2, Anish Sen Majumdar4* and Anjan Kumar Das1*

Author Affiliations

1 Stempeutics Research Malaysia Sdn. Bhd, Technology Park Malaysia, 57000, Kuala Lumpur, Malaysia

2 Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India

3 Hematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

4 Stempeutics Research Pvt. Ltd, Akshay Tech Park, Whitefield, Bangalore, 560066, India

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Journal of Translational Medicine 2012, 10:229  doi:10.1186/1479-5876-10-229

Published: 21 November 2012

Abstract

Background

Numerous preclinical and clinical studies have investigated the regenerative potential and the trophic support of mesenchymal stem cells (MSCs) following their injection into a target organ. Clinicians favor the use of smallest bore needles possible for delivering MSCs into vascular organs like heart, liver and spleen. There has been a concern that small needle bore sizes may be detrimental to the health of these cells and reduce the survival and plasticity of MSCs.

Methods

In this report, we aimed to investigate the smallest possible bore size needle which would support the safe delivery of MSCs into various tissues for different clinical or cosmetic applications. To accomplish this we injected cells via needle sizes 24, 25 and 26 G attached to 1 ml syringe in the laboratory and collected the cells aseptically. Control cells were ejected via 1 ml syringe without any needle. Thereafter, the needle ejected cells were cultured and characterized for their morphology, attachment, viability, phenotypic expression, differentiation potential, cryopreservation and in vivo migration abilities. In the second phase of the study, cells were injected via 26 G needle attached to 1 ml syringe for 10 times.

Results

Similar phenotypic and functional characteristics were observed between ejected and control group of cells. MSCs maintained their cellular and functional properties after single and multiple injections.

Conclusions

This study proves that 26 G bore size needles can be safely used to inject MSCs for clinical/therapeutics purposes.

Keywords:
Bone Marrow Mesenchymal stem cells (BM-MSC); Differentiation; Cell migration needle bore size; MSC transplantation/infusion