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Open Access Research

Chronic renoprotective effect of pulsatile perfusion machine RM3 and IGL-1 solution in a preclinical kidney transplantation model

Raphael Thuillier1234, Ricardo Codas34, Etienne Marchand5, Hubert Lathelize6, Olivier Page6, Alexandre Valagier7, Lionel Badet13789 and Thierry Hauet1101123*

Author Affiliations

1 Inserm U1082, Université de Poitiers, Faculté de Médecine et Pharmacie, Poitiers, 86021, France

2 Service de Biochimie, CHU de Poitiers, Poitiers, F-86021, France

3 FLIRT: Fédération pour L’étude de l’Ischémie Réperfusion en Transplantation, Poitiers, F-86000, France

4 Service d'Urologie et chirurgie de la transplantation, Pavillon V - Hôpital Edouard Herriot, 5, place d’Arsonval, Lyon, F-69437, France

5 CHU Trousseau, Service de chirurgie vasculaire, Fédération hospitalière Tours-Poitiers, Saint-Avertin, F-37550, France

6 Service de Chirurgie Vasculaire, CHU de Poitiers, Poitiers, F-86021, France

7 Faculté de Médecine Lyon-Sud, Oullins Cedex, Oullins, 69921, France

8 University Claude Bernard Lyon 1, Villeurbanne cedex, Villeurbanne, F-69622, France

9 Réseau CENTAURE, 63, quai Magellan, Nantes, F-44000, France

10 Plate forme IBiSA, domaine du Magneraud, BP 52, Saint Pierre d’Amilly, Surgères, F-17700, France

11 Inserm U1082, 2 rue de la miletrie BP577, Poitiers, 86021, France

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Journal of Translational Medicine 2012, 10:233  doi:10.1186/1479-5876-10-233

Published: 21 November 2012

Abstract

Background

Machine perfusion (MP) of kidney graft provides benefits against preservation injury, however decreased graft quality requires optimization of the method. We examined the chronic benefits of MP on kidney grafts and the potential improvements provided by IGL-1 solution.

Method

We used an established autotransplantation pig kidney model to study the effects of MP against the deleterious effects of warm ischemia (WI: 60 minutes) followed by 22 hours of cold ischemia in MP or static cold storage (CS) followed by autotransplantation. MPS and IGL-1 solutions were compared.

Results

Animal survival was higher in MPS-MP and both IGL groups. Creatinine measurement did not discriminate between the groups, however MPS-MP and both IGL groups showed decreased proteinuria. Chronic fibrosis level was equivalent between the groups. RTqPCR and immunohistofluorescent evaluation showed that MP and IGL-1 provided some protection against epithelial to mesenchymal transition and chronic lesions. IGL-1 was protective with both MP and CS, particularly against chronic inflammation, with only small differences between the groups.

Conclusion

IGL-1 used in either machine or static preservation offers similar levels of protection than standard MP. The compatibility of IGL-1 with both machine perfusion and static storage could represent an advantage for clinical teams when choosing the correct solution to use for multi-organ collection. The path towards improving machine perfusion, and organ quality, may involve the optimization of the solution and the correct use of colloids.