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This article is part of the supplement: 7th European Workshop on Immune-Mediated Inflammatory Diseases

Open Access Invited speaker presentation

Finding new immune regulatory genes by ENU mutagenesis

Anselm Enders1*, Hannes Bergmann1, Mehmet Yabas1, T Dan Andrews23, Belinda Whittle3, Mathew Field23, Bhavani Balakishnan3, Yovina Sontani2, Lisa Miosge2, Geoff Sjollema3, Stuart Read3, Ed Bertram23 and Chris C Goodnow2

  • * Corresponding author: Anselm Enders

Author Affiliations

1 Rammaciotti Immunisation Genomics Laboratory, John Curtin School of Medical Research, Australian National University, Canberra, Australia

2 Immunogenomics Laboratory, John Curtin School of Medical Research, Australian National University, Canberra, Australia

3 Australian Phenomics Facility, Australian National University, Canberra, Australia

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Journal of Translational Medicine 2012, 10(Suppl 3):I6  doi:10.1186/1479-5876-10-S3-I6


The electronic version of this article is the complete one and can be found online at: http://www.translational-medicine.com/content/10/S3/I6


Published:28 November 2012

© 2012 Enders et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction

The Thousand Genomes Project has revealed the extraordinary scale of human genetic variation such as each person inherits ~12,000 protein-changing single nucleotide variations (SNVs) including up to 100 premature STOP codons creating an immense challenge to investigate the physiological consequence of this type of genetic variation in experimental animals.

Aim

To develop a new strategy to meet this need by using whole exome capture, massively parallel DNA sequencing and computational analysis to sensitively and specifically identify 40-50 de novo mis-sense SNV mutations spread across the genome of individual offspring from ENU-mutagenized C57BL/6 mice [1].

Results

In this presentation we demonstrate how direct sequencing of animals with independent defects in B cell development resulted in the identification of the causative mutation both in genes of previously known and unknown function without meiotic mapping. This approach has resulted in the identification of a new phospholipid transport pathway that is crucial for normal B cell development in the bone marrow [2] and the realization that an endopeptidase of previously unknown in vivo function is essential for normal processing of MHC invariant chain and terminal B cell and DC maturation.

By sequencing animals from defined strains and also founder animals of ENU mutant pedigrees two generations before the phenotypic screens we have started to build a database of missense mutations containing currently close to 7000 mutations in mouse pedigrees actively breeding and immediately available for experimental analysis. All data is made available from the missense variant database (pb.apf.edu.au/phenbank).

Conclusion

This approach transforms mammalian experimental genetics and opens up an unparalleled source for experimental models of human disease and validation of human disease associated SNVs.

References

  1. Andrews TD, Whittle B, Field MA, Balakishnan B, Zhang Y, Shao Y, Cho V, Kirk M, Singh M, Xia Y, Hager J, Winslade S, Sjollema G, Beutler B, Enders A, Goodnow CC: Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models.

    Open Biol 2012, 2:120061. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Yabas M, Teh CE, Frankenreiter S, Lal D, Roots CM, Whittle B, Andrews DT, Zhang Y, Teoh NC, Sprent J, Tze LE, Kucharska EM, Kofler J, Farell GC, Bröer S, Goodnow CC, Enders A: ATP11C is critical for the internalization of phosphatidylserine and differentiation of B lymphocytes.

    Nat Immunol 2011, 12:441-449. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL