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Roles of CXCL5 on migration and invasion of liver cancer cells

Xiaojing Xu1, Peixin Huang1, Biwei Yang1, Xiangdong Wang2* and Jinglin Xia1*

Author Affiliations

1 Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China

2 Department of Pulmonary Medicine, The First Hospital of Wenzhou Medical University, Wenzhou, China

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Journal of Translational Medicine 2014, 12:193  doi:10.1186/1479-5876-12-193

Published: 10 July 2014


Inflammatory factors play a vital role in the progression of liver cancer, although exact factors and related mechanisms still remain unclear. The present study aimed at screening inflammatory factors related to liver cancer metastasis and investigating the potential mechanism by which cancer cells are recruited. We screened and validated inflammatory factors by microarray and RT-PCR. Small interfering RNA (siRNA) and recombinant protein were used to assess CXCL5 effects on the movement of liver cancer cells (LCCs). Our screening microarray demonstrated over-expression of CXCL5 in LCCs with high metastatic potentials. CXCL5 increased LCCs migration and invasion, probably through autocrine and paracrine mechanisms. CXCL5-CXCR2 and ERK1/2 pathways could play critical roles in the regulation of LCCs migration. Our data indicates that LCCs per se may act as the producer and receptor of CXCL5 responsible for liver cancer migration and invasion.

CXCL5; CXCR2; Migration; Invasion; Liver cancer