Figure 6.

Suppressive Effects of DNA vaccine to Tumor Growth. C57BL/6 mice (n= 6/group) were vaccinated with pCDNA3.1-PSMA or empty vector, and then challenged with B16-PSMA cells. Kaplan-Meier curves showed the tumor-free survival time of mice was 19.67 ± 2.24 days in DNA vaccine group, 22.33 ± 1.61 days in CpG + DNA and 13.17 ± 1.01 days in control group respectively (Fig. 6A). Some mice still developed tumors after pCDNA3.1-PSMA and CpG oligodeoxynucleotides vaccination, but the individual tumors grew much more slowly than those in control group (Fig. 6B). Two mice in control group died before the termination of the experiment, all mice bearing tumor were sacrificed and tumor tissues were removed, the volumes of tumors in control group were larger than those in other two groups (Fig. 6C).