Figure 1.

Granzyme B release by stimulated TALL-104 cells measured in the ELISPOT assay. TALL-104 cells (1 × 10³, 2 × 10³ or 5 × 10³ cells per well) were incubated with K562 targets (5 × 10⁴ cells per well) for 4 h at 37°C in the Granzyme B ELISPOT assay (effector:target cell ratios are in brackets). Data are presented as average number of spots per well ± SD and are representative of 3 experiments with similar results. A significant correlation between the number of spots per well and effector cell number was observed (R² = 0.98).