Recognition of peptides and tumor cells by TIL 5 TCR-transduced CMV-stimulated T cells. TIL 5 TCR-transduced and untransduced CMV-stimulated bulk cultures were cocultured for 24 hours in a 1:1 ratio with T2 cells loaded with 5 μg/ml of peptide, COS A2 cells with or without a CMV minigene, or A2+, MART-1+ tumor cells (SK23 MEL, 624 MEL), A2-, MART-1+ tumor cells (624.28 MEL), or A2+, MART-1- tumor cells (UOK131 RCC). Supernatants were collected and the amount of interferon-γ released was measured using ELISA. Values are the average of triplicate wells.
Langerman et al. Journal of Translational Medicine 2004 2:42 doi:10.1186/1479-5876-2-42