Long term maintenance of bifunctionality in culture. TIL 5 TCR-transduced cultures were cocultured for 24 hours in a 1:1 ratio with T2 cells pulsed with 5 μg/ml peptide at varying time points beyond transduction. Supernatants were collected and the amount of interferon-γ released was measured using ELISA. Values are the average of triplicate wells. Asterisk (*) indicates value was greater than maximum point on standard curve.
Langerman et al. Journal of Translational Medicine 2004 2:42 doi:10.1186/1479-5876-2-42