Peptide recognition by TIL 5 TCR-transduced gp100-reactive T cell clone. TIL 5 TCR-transduced and untransduced R6C12 cells were cocultured for 24 hours in a 1:1 ratio with T2 cells loaded with 5 μg/ml of peptide. Supernatants were collected and the amount of interferon-γ released was measured using ELISA. Values are the average of duplicate wells.
Langerman et al. Journal of Translational Medicine 2004 2:42 doi:10.1186/1479-5876-2-42