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Transwell Experiments |
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| 3H-TDR (CPM) |
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| *Immature CD56 |
**Mature CD56 |
***K562 |
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| Exp 1 |
6781 ± 51 |
5941 ± 560 |
4429 ± 197 |
| Exp 2 |
3116 ± 65 |
2534 ± 196 |
4030 ± 239 |
| Exp 3 |
6187 ± 512 |
2932 ± 181 |
6873 ± 34 |
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Sorted Immature * or mature CD56+ cells** or K562 were incubated 10:1 ratio in the upper chamber at of Transwell plates while K562 were incubated in the lower chamber. After 2 days, lower K562 were collected labeled overnight with 3H-TDR and analyzed in a β-counter 0.4 μm polycarbonate transwell membrane were removed and the upper compartment cells were harvested and evaluated for viability by trypan blue exclusion assay. | |||
Sconocchia et al. Journal of Translational Medicine 2005 3:15 doi:10.1186/1479-5876-3-15 |
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