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Cytoplasmic markers expressed by primary cultures of skeletal muscle cells grown in vitro for 35 to 40 days1. |
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| Monoclonal antibody |
Percentage of positive cells (mean ± SD)2 |
Fluorescence pattern |
|
|
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| Desmin (skeletal muscle) |
49.6 + 6.4 |
Cytoplasmic |
| Skeletal myosin type I slow heavy chain (skeletal and cardiac muscle) |
33.4 + 3.9 |
Cytoplasmic filaments |
| A-sarcomeric actin (skeletal and cardiac muscle) |
28.6 + 6.1 |
Cytoplasmic filaments |
| Skeletal myosin type II fast heavy chain (skeletal muscle, not cardiac) |
29.4 + 6.2 |
Cytoplasmic filaments |
| A-sarcomeric actinin (skeletal and cardiac muscle) |
19.0 + 3.7 |
Cytoplasmic filaments |
| Spectrin (erythrocyte and muscle) |
32.6 + 7.5 |
Peripheral rim and membrane staining |
| Dystrophin (skeletal, cardiac and smooth muscle) |
38.4 + 9.8 |
Peripheral rim and membrane staining |
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1. Indirect immunofluorescence assay on monolayers of cells grown using proliferation medium. 2. Positive cells in primary cultures derived from five different tissue donors. | ||
Baj et al. Journal of Translational Medicine 2005 3:21 doi:10.1186/1479-5876-3-21 |
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