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Open Access Highly Accessed Research

The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects

DL Mager1*, AD Haffajee1, PM Devlin2, CM Norris3, MR Posner3 and JM Goodson1

Author Affiliations

1 The Forsyth Institute, 140 The Fenway, Boston, MA, USA

2 Brigham and Women's Hospital, 27 Francis Street, Boston, MA, USA

3 Dana Farber Cancer Institute, 44 Binney Street, Boston, MA, USA

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Journal of Translational Medicine 2005, 3:27  doi:10.1186/1479-5876-3-27

Published: 7 July 2005

Abstract

Background

The purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (OSCC) lesion would differ from those found in cancer-free (OSCC-free) controls.

Methods

Unstimulated saliva samples were collected from 229 OSCC-free and 45 OSCC subjects and evaluated for their content of 40 common oral bacteria using checkerboard DNA-DNA hybridization. DNA counts per ml saliva were determined for each species, averaged across subjects in the 2 subject groups, and significance of differences between groups determined using the Mann-Whitney test and adjusted for multiple comparisons. Diagnostic sensitivity and specificity in detection of OSCC by levels of salivary organisms were computed and comparisons made separately between a non-matched group of 45 OSCC subjects and 229 controls and a group of 45 OSCC subjects and 45 controls matched by age, gender and smoking history.

Results

Counts of 3 of the 40 species tested, Capnocytophaga gingivalis, Prevotella melaninogenica and Streptococcus mitis, were elevated in the saliva of individuals with OSCC (p < 0.001). When tested as diagnostic markers the 3 species were found to predict 80% of cancer cases (sensitivity) while excluding 83% of controls (specificity) in the non-matched group. Diagnostic sensitivity and specificity in the matched group were 80% and 82% respectively.

Conclusion

High salivary counts of C. gingivalis, P. melaninogenica and S. mitis may be diagnostic indicators of OSCC.

Keywords:
Oral Squamous Cell Carcinoma; Oral mucosa; bacterial markers; bacteria; early detection