Table 2

Gene expression studies: dissected aorta ascendens vs. control aorta. Gene expression studies that comparing freshly dissected aorta ascendens (D) and undissected control aorta (C). 3 different gene expression studies were performed: 16 samples were studied with BD Atlas™ Human1.2 Array (h), 16 samples – with BD Atlas™ Human Cardiovascular Array (c) and 8 samples – with Affymetrix Human Genome U133A GeneChip. All differently expressed genes of the Atlas-experiments, 49 genes (h) and 33 genes (c), are shown and compared to genes of the Affymetrix experiment. Ratio: mean expression of dissected aorta (D) divided by mean expression of control (C); n.e.: not expressed, n.s.: difference not significant. n.t.: not tested, ↓ downregulated, ↑ upregulated; ? – regulation in the Atlas and Affymetrix experiment different
Gene Name
 Platform
 Atlas
 Affymetrix
 regulation
Functional group
 Array type
 ratio D/C
 P value
 ratio D/C
 P value
extracellular matrix proteins,





cell surface antigens, adhesion proteins





collagen IV α2, COL4A2
 h
 0.5
 <0.01
 0.4
 <0.05
 ↓
collagen IV α5, COL4A5
n.t.
0.32
 <0.05
 ↓
collagen VI α3, COL6A3
 c
 1.9
 <0.05
n.s.
 ?
collagen VIII α1, COL8A1
 c
 0.3
 <0.01
 0.3
 <0.001
 ↓
collagen VIII α2, COL8A2
n.t.
0,4
 <0,01
 ↓
collagen XIV α1, COL14A1, undulin
 c
 0.4
 <0.001
 0.5
 <0.001
 ↓
collagen XVIII α1, COL18A1
n.t.
0.47
 <0.001
 ↓
polycystin1, PKD1
 h
 0.3
 <0.001
 0.4
 <0.01
 ↓
polycystin 2, PKD2
n.t.
0.4
 <0.001
 ↓
clusterin, SP-40;
 h
 0.5
 <0.01
 0.5
 <0.01
 ↓
integrin α7B, IGA7B
 h
 0.4
 <0.001
 0.3
 <0.001
 ↓
gap junction protein, connexin 43
 c
 0.6
 <0.05
n.s.
 ?
SPARC-like 1
 c
 0.3
 <0.001
 0.6
 <0.001
 ↓
decorin
 c
 0.3
 <0.05
n.s.
 ↓
elastin
n.t.
0.4
 <0.05
 ↓
fibulin 5
n.t.
0.4
 <0.001
 ↓
cell surface glycoprotein MUC18
 h
 0.3
 <0.001
 0.5
 <0.01
 ↓
fibronectin receptor
 h
 n.s.
3.4
 <0.01
 ↑
ras homolog gene family member B
 h
 0.5
 <0.05
n.s.
 ?
extracellular matrix proteolysis





MMP-11
 h
 2.1
 <0.001
 3.2
 <0.05
 ↑
MMP-14
n.t.
2.5
 <0.01
 ↑
MMP-19
n.t.
3.2
 <0.05
 ↑
TIMP1
 h,c
 1.8
 <0.01
n.s.
 ?
TIMP2
 h,c
 0.4
 <0.01
n.s.
 ?
TIMP3
 c
 0.2
 <0.001
n.s.
 ?
cell motility and cytoskeleton proteins





vinculin
 c
 0.2
 <0.001
n.s.
 ?
actinin α1
 c
 0.5
 <0.001
 0.8
 <0.01
 ↓
filamin A
 c
 0.3
 <0.001
 0.5
 <0.001
 ↓
actin α2
n.t.
0.13
 <0.001
 ↓
actinin α4
n.t.
0.44
 <0.001
 ↓
gelsolin
n.t.
0.5
 <0.01
 ↓
leiomodin
n.t.
0.4
 <0.01
 ↓
tropomyosin 1
n.t.
0.4
 <0.01
 ↓
calponin
n.t.
0.3
 <0.001
 ↓
smooth & non-muscle myosin light chain kinase
 h
 0.4
 <0.001
 n.t.
↓
smooth muscle myosin heavy chain, SM2
n.t.
0.5
 <0.001
 ↓
myosin regulatory light chain2, smooth muscle
n.t.
0.5
 <0.001
 ↓
metabolism
n.t.



low-density lipoprotein receptor, LDLR
 c
 0.4
 <0.001
 1.6
 <0.01
 ?
Kunitz-type serine protease inhibitor 2, SPINT2
 h
 0.2
 <0.001
 0.3
 <0.001
 ↓
aldehyde dehydrogenase 2, mitochondrial
 c
 0.3
 <0.01
 0.6
 <0.01
 ↓
diaphorase (NADH), cytochrome b-5 reductase
 c
 0.5
 <0.01
n.s.
 ?
superoxide dismutase 3, extracellular
 c
 0.4
 <0.01
 0.4
 <0.001
 ↓
lipase A
 c
 2.6
 <0.05
n.s.
 ?
protease inhibit, PI1, alpha-1-antitrypsin
 h
 5.5
 <0.001

↑
GABA-B receptor 1A subunit
 h
 1.8
 <0.001
 n.e.
?
prostaglandin I2 prostacyclin synthetase
 c
 0.4
 <0.05
 0.81
 <0,001
 ↓
Gene Name
 Platform
 Atlas
 Affymetrix
 regulation
Functional group
 Array type
 ratio D/C
 P value
 ratio D/C
 P value
 ratio D/C
Protein turnover





purine-rich element-binding protein A
 h
 0.4
 <0.01
 0.6
 <0.05
 ↓
glutathione S-transferase theta 1
 h
 0.4
 <0.01
 0.2
 <0.05
 ↓
58-kDa inhibit. of RNA-activated protein kinase
 h
 0.3
 <0.01

↓
G1/S-specific cyclin D1
 h
 0.5
 <0.01
n.s.
 -
carboxypeptidase H, CPE; enkephalin convertase
 h
 0.4
 <0.01

↓
ribos. protein S6 kinase II α1, S6KII-alpha 1
 h
 3.1
 <0.001
n.s.
 -
carboxypeptidase N, polypeptide 1, 50 kD
 c
 2.0
 <0.05
 n.e.
-
eukariotic translation initiantion factor 4E bind prot 1
n.t.
2.9
 <0.01
 ↑
60 S acdic ribosomal protein PO
n.t.
3.0
 <0.01
 ↑
FOS-related antigen 1
 h,c
 4.9
 <0.01
 3.6
 <0.05
 ↑
myc proto-oncogene
 h
 3.5
 <0.01
 2.8
 <0.05
 ↑
ets domain protein elk-3; NET; SRF accessory protein
 h
 2.1
 <0.001
 4.4
 <0.05
 ↑
adenine phosphoribosyltransferase
n.t.
2.1
 <0.001
 ↑
high mobility group prot isoforms I & Y, HMGIY
 h
 10.1
 <0.001
 6,0
 <0.01
 ↑
superoxide dismutase 2, mitochondrial
 c
 2.6
 <0.01
 3.7
 <0.001
 ↑
Cell receptors





Natriuretic peptide receptor A/guanylate cyclase A
 c
 0.4
 <0.001
 0.3
 <0.001
 ↓
natriuretic peptide receptor C/guanylate cyclase C
 c
 0.5
 <0.001
n.s.
 -
protein tyrosine phosphatase receptor type F
 c,h
 0.4
 <0.01
 n.t.
-
growth factors





vascular endothelial growth factor, VEGF
 h
 4.7
 <0.05
n.s.
 -
endothelin 2
 c
 0.6
 <0.01
 n.e.
-
fibroblast growth factor receptor1
 h
 0.6
 <0.01
 0.7
 <0.05
 ↓
insulin-like growth factor-binding protein 2, IGFBP2
 h
 0.3
 <0.001
 0.2
 <0.01
 ↓
cardiotrophin 1
 c
 0.4
 <0.001
 0.5
 >0.05
 ↓
pleiotrophin, heparin-binding growth factor 8, HBGF8
 h
 0.5
 <0.05
 0.6
 <0.05
 ↓
inflammation + stress response





colony-stimulating factor 1 receptor, CSF1R
 h
 2.0
 <0.05
 1.7
 <0.05
 ↑
myeloid cell nuclear differentiation antigen
 h
 3.5
 <0.001
 3.9
 <0.05
 ↑
tumor necrosis fact recept superfam memb 1B
 h
 2.0
 <0.001
n.s.
 -
granulocyte colony stimul factor recept
 h
 3.5
 <0.01
 n.e.
-
interleukin 2 receptor alpha subunit, IL-2, CD25 antigen
 h
 3.1
 <0.001
 5.3
 <0.001
 ↑
interleukin 6, IL-6
 h
 3.9
 <0.01
 4.3
 <0.001
 ↑
interleukin 8, IL-8


12.2
 <0.05
 ↑
small inducible cytokine subfamily A member 2
 h,c
 3.8
 <0.01
 2.2
 <0.05
 ↑
90-kDa heat-shock proteinA HSP90A
 h
 2.6
 <0.05
 n.t.
↑
60-kDa heat shock protein HSP60
 h
 2.7
 <0.05
 n.t.
↑
CD9 antigen p24
 c
 0.5
 <0.001
 0.5
 <0.01
 ↓
27-kDa heat shock protein HSP27
 h
 0.4
 <0.001
 n.t.
-
blood, hemostasis





endothelial plasminogen activator inhibit 1, PAI1
 h
 2.8
 <0.05
 4.0
 <0.05
 ↑
thrombomodulin
 h
 2.8
 <0.05
n.s.
 -
hexabrachion, tenascin C
 c
 2.6
 <0.01
 6.2
 <0.05
 ↑
heme oxygenase 1
 h
 15.4
 <0.05
 3.6
 <0.05
 ↑
tissue factor pathway inhibit, coagulation inhibit
 c
 2.0
 <0.05
 1.6
 <0.05
 ↑
tissue factor pathway inhibitor 2
 c
 2.3
 <0.05
 3.0
 <0.05
 ↑
others





c-fgr proto-oncogene p55-FGR
 h
 2.6
 <0.01
 2.6
 <0.01
 ↑
erbB3 proto-oncogene; HER3
 h
 3.7
 <0.001
 n.t.
↑
caspase 4
 h
 1.9
 <0.05
 n.t.
↑
follistatin-related protein
 h
 0.5
 <0.01
 0.6
 <0.01
 ↓
Gem; induced immediate early protein
 h
 0.2
 <0.001
 0.3
 <0.001
 ↓
parathymosin athymosin
 h
 0.6
 <0.05
 0.8
 n.s.
 -
prostaglandin I2 (prostacyclin) synthase
 c
 0.4
 <0.05
 n.t.
 <0.001
 ↓
calcium-activated potassium channel beta subunit
 h
 0.3
 <0.001
 0.4
 <0.01
 ↓
solute carrier fam 4 anion exchanger member 3
 c
 0.5
 <0.001
 n.t.
-

Weis-Müller et al. Journal of Translational Medicine 2006 4:29   doi:10.1186/1479-5876-4-29