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Open Access Research

Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems

Patrizia Galeffi1*, Alessio Lombardi1, Immacolata Pietraforte15, Flavia Novelli1, Monica Di Donato1, Maria Sperandei1, Andrea Tornambé1, Rocco Fraioli2, Aline Martayan2, Pier Giorgio Natali2, Maria Benevolo3, Marcella Mottolese3, Francisco Ylera4, Cristina Cantale1 and Patrizio Giacomini2*

Author Affiliations

1 ENEA BIOTEC-GEN, CR Casaccia Via Anguillarese 301, 00060 Rome, Italy

2 Laboratory of Immunology, Regina Elena Cancer Institute CRS, Via delle Messi d'Oro 156, 00158 Rome, Italy

3 Laboratory of Pathology, Regina Elena Cancer Institute, Istituti Fisioterapici Ospitalieri, Via E. Chianesi 53, 00144 Rome, Italy

4 Roche Diagnostics GmbH, Nonnenwald 2, D-82372 Penzberg, Germany

5 Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy

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Journal of Translational Medicine 2006, 4:39  doi:10.1186/1479-5876-4-39

Published: 29 September 2006

Abstract

Background

Aberrant signaling by ErbB-2 (HER 2, Neu), a member of the human Epidermal Growth Factor (EGF) receptor family, is associated with an aggressive clinical behaviour of carcinomas, particularly breast tumors. Antibodies targeting the ErbB-2 pathway are a preferred therapeutic option for patients with advanced breast cancer, but a worldwide deficit in the manufacturing capacities of mammalian cell bioreactors is foreseen.

Methods

Herein, we describe a multi-platform approach for the production of recombinant Single chain Fragments of antibody variable regions (ScFvs) to ErbB-2 that involves their functional expression in (a) bacteria, (b) transient as well as stable transgenic tobacco plants, and (c) a newly developed cell-free transcription-translation system.

Results

An ScFv (ScFv800E6) was selected by cloning immunoglobulin sequences from murine hybridomas, and was expressed and fully functional in all the expression platforms, thereby representing the first ScFv to ErbB-2 produced in hosts other than bacteria and yeast. ScFv800E6 was optimized with respect to redox synthesis conditions. Different tags were introduced flanking the ScFv800E6 backbone, with and without spacer arms, including a novel Strep II tag that outperforms conventional streptavidin-based detection systems. ScFv800E6 was resistant to standard chemical radiolabeling procedures (i.e. Chloramine T), displayed a binding ability extremely similar to that of the parental monovalent Fab' fragment, as well as a flow cytometry performance and an equilibrium binding affinity (Ka approximately 2 × 108 M-1) only slightly lower than those of the parental bivalent antibody, suggesting that its binding site is conserved as compared to that of the parental antibody molecule. ScFv800E6 was found to be compatible with routine reagents for immunohistochemical staining.

Conclusion

ScFv800E6 is a useful reagent for in vitro biochemical and immunodiagnostic applications in oncology, and a candidate for future in vivo studies.