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Panel A: List of the biologically active factors released by activated platelets. Panel B: Protocol for the preparation of PRP in each bioassay. The amount of blood collected, the centrifugal force, and the final PRP volume are shown. |
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| Panel A |
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| Biologically active factors released by the activated platelets11–15 |
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| ADP, ATP, Serotonin, Platelet-derived Growth Factor (PDGF), Transforming Growth Factor-β1 (TGF-β1), Connective Tissue Activating Peptide III (CATP III), Insuline-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), Angiopoietin-2 (Ang-2), Thrombospondin, Factor V, Factor XI, Factor XIII, Platelet-derived endothelial cell growth factor (PDECGF), Osteocalcin, and adhesive proteins such as Fibrinogen, Fibronectin, von Willebrand Factor (vWF), and P-Selectin. |
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| Panel B |
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| Bioassay |
Collected Blood (final volume, including anticoagulant) |
Collection site |
First spinning (RCF) |
Second spinning (RCF) |
PRP volume |
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| Heterotopic bioassay (Ferret) |
5 ml |
Jugular vein |
40 g for 20' |
235 g for 10' |
250 μl |
| Orthotopic bioassay (Rat) |
3 ml |
Jugular vein |
380 g for 2'15" |
380 g for 5' |
75 μl |
| Orthotopic bioassay (Non-human primate) |
30 ml |
Cephalic vein |
1130 g for 2'15" |
1130 g for 5' |
1.5 ml |
| Clinical cases (Homo sapiens) |
20 ml |
Cephalic vein Basilic vein Median cubital vein |
1130 g for 2'15" |
1130 g for 5' |
1 ml |
Intini et al. Journal of Translational Medicine 2007 5:13 doi:10.1186/1479-5876-5-13 |
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