Table 1

In vivo compound treatments used in training and testing

Compound
Class
Conducted by
Dose (mpk)
Necropsy day
Vehicle – Route

Cisplatin
DNA – alkylator
Merck
0.5
3, 8
0.9% (w/v) sodium chloride – IP



3.5
3, 8




7
3, 8

Cyclosporin A
Calcineurin inhibitor
Merck
6
3, 9, 15
olive oil – SC



30
3, 9, 15




60
3, 9, 15

Gentamycin
Antibiotic
Merck
20
3, 9, 15
0.9% (w/v) sodium chloride – IP



80
3, 9, 15




240
3, 9, 12

Sodium Fluoride
Environmental toxin
Merck
35
3, 8, 12
Water – PO



75
3, 8, 12

Merck X
Antibiotic
Merck
75
3, 8, 14
0.5%saline – IV



150
3, 8, 14




225
3, 8

Allopurinol
Xanthine oxidase inhibitor
Charles River
6
3
corn oil – IP



30
3




100
3, 7, 14

D-serine
Serine analog
Charles River
750
3, 14
water – IP
Hexachloro 1,3, butadiene
Synthetic toxin
Charles River
7.5
3
corn oil – IP



40
3, 14




100
3

Puromycin
Antibiotic
Charles River
5
3
0.9% (w/v) sodium chloride – IP



20
3, 7, 14




60
3, 7

Tobramycin
Antibiotic
Charles River
6
3
0.9% (w/v) sodium chloride – IP



30
14




60
3, 14


Male Sprague-Dawley rats were treated daily with the listed compounds except D-serine which was given as a single dose once on day 0. Each dose group includes 4 or 5 rats. Animals were terminated at the end of study. Terminal or interim necropsy were performed 24 hours post dosing. Kidney expression profiles were obtained for each necropsy day (when kidney samples were harvested). Appropriate dosing routes were applied: PO – Oral Garvage, IV – intravenous, SC – subcutaneous.

Jiang et al. Journal of Translational Medicine 2007 5:47   doi:10.1186/1479-5876-5-47