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Endometrial regenerative cells: A novel stem cell population

Xiaolong Meng1*, Thomas E Ichim2, Jie Zhong1, Andrea Rogers1, Zhenglian Yin1, James Jackson1, Hao Wang3, Wei Ge3, Vladimir Bogin2, Kyle W Chan2, Bernard Thébaud4 and Neil H Riordan12

Author Affiliations

1 Bio-Communications Research Institute, Wichita, USA

2 Medistem Laboratories Inc, Tempe, USA

3 Department of Surgery, University of Western Ontario, London, Canada

4 Department of Pediatrics, University of Alberta, Edmonton, Canada

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Journal of Translational Medicine 2007, 5:57  doi:10.1186/1479-5876-5-57

Published: 15 November 2007

Abstract

Angiogenesis is a critical component of the proliferative endometrial phase of the menstrual cycle. Thus, we hypothesized that a stem cell-like population exist and can be isolated from menstrual blood. Mononuclear cells collected from the menstrual blood contained a subpopulation of adherent cells which could be maintained in tissue culture for >68 doublings and retained expression of the markers CD9, CD29, CD41a, CD44, CD59, CD73, CD90 and CD105, without karyotypic abnormalities. Proliferative rate of the cells was significantly higher than control umbilical cord derived mesenchymal stem cells, with doubling occurring every 19.4 hours. These cells, which we termed "Endometrial Regenerative Cells" (ERC) were capable of differentiating into 9 lineages: cardiomyocytic, respiratory epithelial, neurocytic, myocytic, endothelial, pancreatic, hepatic, adipocytic, and osteogenic. Additionally, ERC produced MMP3, MMP10, GM-CSF, angiopoietin-2 and PDGF-BB at 10–100,000 fold higher levels than two control cord blood derived mesenchymal stem cell lines. Given the ease of extraction and pluripotency of this cell population, we propose ERC as a novel alternative to current stem cells sources.