Figure 3.

Serial dilutions of CCRF-CEM (Vβ9) and MOLT-16 (Vβ2, Vβ18) cell lines in PBMCs of a healthy volunteer. Measured percentages of all families in case of 50, 500, 5000, 50 000 CCRF-CEM cells (A) or MOLT-16 cells (B) added to 100 000 PBMCs of volunteer 2. Dilutions, RNA extraction, cDNA synthesis and qRT PCR are performed in duplicated. Mean percentages of each dilution step were shown. P_j: Vβ-chain relative expression.