Table 2

Impact of temperature on AdCCL21 transduction of DC.
AdCCL21-DC
Recovery
 Viability
 CCL21
37°C
 RT
 37°C
 RT
 37°C
 37°C




ng/1 × 106
 ng/1 × 106
 ng/1 × 106
 Ng/1 × 106
%
 24 h
 48
 24 h
 48
50.7 ± 14
 59 ± 0.03
 75 ± 0.2
 *93.6 ± 0.02
 1.1 ± 0.7
 1.9 ± 1.3
 2.9 ± 2.1
 3.7 ± 2.5

• p = 0.05

In preliminary experiments (n = 4), the impact of temperature on adenoviral transduction of human DC was evaluated. Cell recovery, viability by trypan blue exclusion, and CCL21 production are shown at 37°C and RT.

Baratelli et al. Journal of Translational Medicine 2008 6:38   doi:10.1186/1479-5876-6-38