Whole genome expression and biochemical correlates of extreme constitutional types defined in Ayurveda

Additional file 1:

Original Sanskrit versions supporting the text. References from original compendium of Ayurveda, pertaining to the concept of Prakriti, Tridosha and its importance in predictive and curative medicine.

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Additional file 2:

Questionnaire for Prakriti evaluation. A detailed questionnaire for evaluation of Prakriti developed on the basis of original Ayurveda text.

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Additional file 3:

Normal reference range for biochemical and hematological parameters. A table of standard accepted normal ranges in males and females for biochemical and hematological parameters.

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Additional file 4:

Neighbor Joining tree showing relatedness of study population to the Indo European population. Heterogeneity and inter-relatedness of Indian populations among themselves and with study population (VPK) depicted in neighbour-joining tree illustrating population affinities based on Nei's DA distance. The study population are genetically closer to the Indo-European large populations predominantly from North India. The symbols represents linguistic Lineage (AA, Austro-Asiatic; IE, Indo-European; DR, Dravidian and TB, Tibeto-Burman) followed by geographical location (N, north; NE, north-east; W, west; E, east; S, south and C, central) and Ethnic category (LP, castes/large populations; SP, religious groups/small populations and IP, tribes/isolated population).

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Additional file 5:

Differences in Biochemical and hematocrit parameters among Prakriti groups in males and females. The data provided shows significant differences (p ≤ 0.05) in biochemical parameters and hematocrit between Prakriti groups in males (Table 2) and females (Table 3) as calculated using ANOVA (for values with normal distribution) and Kruskal Wallis (for values with non-normal distribution).

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Additional file 6:

Validation of biochemical differences amongst three Prakriti groups through analysis of significance performed in 1000 random iterations of same parent dataset in males. The boxplot of F-values of 1000 randomized comparisons of (A)Total cholesterol, (B) Triglycerides, (C) VLDL, (D) LDL, (E) LDL/HDL, (F) GGPT, (G) Serum Uric acid,(H) Prothrombin Time, (I) Hemoglobin, (J) PCV, (K) RBC, (L) Serum Zinc values. Thick bar indicated median F-value. Red spot is the mean F-value of randomized comparisons and green spot is the comparative mean F-value of V, P, K comparisons.

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Additional file 7:

List of genes differentially expressed among Prakriti types. The data represents a list of differentially expressed genes in males and females with information on their disease association as obtained from OMIM and GAD and their categorization as HUB and/Or Housekeeping genes.

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Additional file 8:

Real time validation of differentially expressed genes from microarray experiments. The data depicts the list of differentially expressed genes validated using quantitative real time PCR with 18S rRNA as internal control. Analysis has been carried out in 96 individual samples and P ≤ 0.05 has been considered significant.

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Additional file 9:

Significantly enriched biological processes which are similarly expressed across Prakriti groups. The table contains significantly enriched (P ≤ 0.01 after Bonferroni correction) biological processes that do not differ among Prakriti groups. Genes which do not differ (as estimated by low S.D) were analysed using GO biological processes. Quantile normalized gene expression values were used to determine SD.

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Additional file 10:

Disease gene network of differentially expressed genes among Prakriti groups. The figure represents networks of differentially expressed genes and their disease associations obtained from Genetic Association Database (GAD) and OMIM in males (A) and females (B). Networks have been depicted using Cytoscape version 2.4.1 http://www.cytoscape.org webcite. The nodes colored in yellow represent disease category and those in pink depict gene symbols. Hub genes are represented by pink square nodes. The most connected genes are placed in the centre of the network.

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