|
Candidate biomarkers for human cancers discovered by cancer secretome analysis |
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| Cancer |
Screening methods |
Verification methods |
Candidate biomarkers |
References |
|
|
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| Lung |
SDS-PAGE/nano-ESI-MS/MS |
ELISA |
CD98, fascin, 14-3-3 η, polymeric immunoglobulin receptor/secretory component |
[73] |
| 2-DE/MALDI-TOF/TOF-MS |
Western blot/ELISA/IHC |
Cathepsin D |
[28] |
|
| 2-DE/MALDI-TOF-MS |
RT-PCR/western blot/ELISA/IHC |
Dihydrodiol dehydrogenase |
[27] |
|
| SDS-PAGE/MALDI-TOF-MS |
ELISA |
L-lactate dehydrogenase B |
[90] |
|
| 2-DE/MALDI-TOF-MS |
RT-PCR/enzyme activity detection |
Mn-SOD |
[29] |
|
| Liver |
LC-MS/MS |
Western blot |
Apolipoprotein E, DJ-1, apolipoprotein H, galectin-3, cathepsin L, cyclophilin A, cystatin C |
[41] |
| Pancreatic |
NuPAGE/LC-MS/MS/SILAC |
Western blot/IHC |
CD9, perlecan, SDF4, apolipoprotein E, fibronectin receptor, Mac-2 binding protein, cathepsin D, cathepsin B, MCP-1, L1CAM |
[25] |
| LC-MS/MS |
RT-PCR/western blot/IHC |
CSPG2/versican, Mac25/angiomodulin |
[43] |
|
| Bladder |
SDS-PAGE/MALDI-TOF-MS |
Western blot |
Pro-u-plasminogen activator |
[91] |
| LC-MS/MS |
CXCL1 |
[92] |
||
| Nasopharyngeal |
SDS-PAGE/MALDI-TOF-MS |
Western blot/ELISA/IHC |
Fibronectin, Mac-2 binding protein, plasminogen activator inhibitor 1 |
[69] |
| Prostate |
LC-MS/MS |
Western blot/ELISA |
Mac-2 binding protein |
[40] |
| Oligonucleotide microarray/genome-based computational prediction |
RT-PCR/ELISA/IHC |
Macrophage inhibitory cytokine 1 |
[22] |
|
| LC-MS/MS |
ELISA |
follistatin, chemokine (C-X-C motif) ligand 16, pentraxin 3, spondin 2 |
[93] |
|
| Melanoma |
NuPAGE/LC-Q-TOF-MS/MS |
Western blot |
Cathepsin D, gp100 |
[79] |
| Breast |
LC-MS/MS |
Western blot |
Galectin-3-binding protein, alpha-1-antichymotrypsin |
[94] |
| LC-MS/MS |
ELISA |
Elafin |
[95] |
|
| Colorectal |
SDS-PAGE/MALDI-TOF-MS |
Q-PCR/Western blot/IHC/ELISA |
Collapsing response mediator protein-2 |
[72] |
| 2-DE/DIGE/MALDI-TOF-MS |
Northern blot/western blot |
Cathepsin D, stratifin, calumenin |
[21] |
|
| Renal |
2-DE/MALDI-TOF-MS/immunoblotting |
Western blot/homogeneous fluorescent immunoassay |
Pro-MMP-7 |
[68] |
| Oral |
SDS-PAGE/MALDI-TOF-MS |
Western blot/IHC/ELISA |
Mac-2 binding protein |
[70] |
| Fibrosarcoma |
Capillary ultrafiltration probe/2-DE/MALDI-TOF-MS |
Cyclophilin A, S100A4, profiling-1, thymosin beta 4, thymosin beta 10, fetuin-A, alpha-1 antitrypsin 1–6, contrapsin, apolipoprotein A-1, apolipoprotein C-1 |
[31] |
|
| Ovarian |
SELDI-TOF MS |
Immunodepletion |
CXC chemokine ligand 1, intact and truncated interleukin 8 |
[66] |
| HPLC fractionation/LC-MS/MS |
Immunoblot/immunofluorescence |
14-3-3 zeta |
[96] |
|
Xue et al. Journal of Translational Medicine 2008 6:52 doi:10.1186/1479-5876-6-52 |
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