Figure 3.

HLA-A24-binding assay of peptides. Binding affinity of peptide to HLA-A24 molecule was evaluated by mean fluorescent intensity (MFI) shift of cell surface HLA-A24 level on T2-A24 cells that were pulsed with each peptide. CMV pp65-derived HLA-A24-binding peptide (QVDPVAALF) and HIV env-derived HLA-A24-binding peptide (RYLRDQQLLGI) were used as positive controls. VSV-derived peptide VSV8 (RGYVYQGL) was used as a negative control. Histograms of MFI shift were displayed for each peptide. MFI shift was calculated as; MFI shift = (MFI of T2-A24 cells pulsed with the peptide) - (MFI of T2-A24 cells without peptide pulsation).