Open Access Research

Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders

Luigi Buonaguro19, Annacarmen Petrizzo1, Marialina Tornesello1, Maria Napolitano2, Debora Martorelli3, Giuseppe Castello2, Gerardo Beneduce4, Amalia De Renzo5, Oreste Perrella6, Luca Romagnoli7, Vitor Sousa7, Valli De Re8, Riccardo Dolcetti3 and Franco M Buonaguro1*

Author Affiliations

1 Lab. of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy

2 Lab of Clinical Immunology, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy

3 Cancer Bio-Immunotherapy Unit, Centro di Riferimento Oncologico, I.R.C.C.S. - National Cancer Institute, Aviano, Italy

4 Clinical Pathology, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy

5 Haematology Unit, University of Naples "Federico II", School of Medicine, Naples, Italy

6 VII Division of Infectious Diseases, Cotugno Hospital, Naples, Italy

7 Areta International, Gerenzano, Italy

8 Experimental and Clinical Pharmacology, Centro di Riferimento Oncologico, I.R.C.C.S. National Cancer Institute, Aviano, Italy

9 Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA

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Journal of Translational Medicine 2010, 8:18  doi:10.1186/1479-5876-8-18

Published: 19 February 2010

Abstract

Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL).

It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy.

In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines.