Open Access Research

Correspondence between salivary proteomic pattern and clinical course in primary Sjögren syndrome and non-Hodgkin's lymphoma: a case report

Chiara Baldini1, Laura Giusti2, Federica Ciregia2, Ylenia Da Valle2, Camillo Giacomelli1, Elena Donadio2, Francesco Ferro1, Sara Galimberti3, Valentina Donati4, Laura Bazzichi1, Stefano Bombardieri1 and Antonio Lucacchini2*

Author Affiliations

1 Department of Internal Medicine, Rheumatology Unit, University of Pisa, Pisa, Italy

2 Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Pisa, Italy

3 Department of Oncology, Transplants and New Medical Techniques, Hematology Unit, University of Pisa, Pisa, Italy

4 Pathological Anatomy Hospital Unit, Pisa, Italy

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Journal of Translational Medicine 2011, 9:188  doi:10.1186/1479-5876-9-188

Published: 2 November 2011

Abstract

Background

In the last years human proteomic has represented a promising tool to promote the communication between basic and clinical science.

Methods

To explore the correspondence between salivary proteomic profile and clinical response, herein, we used a proteomic approach to analyse the whole saliva of a patient with primary Sjögren's Syndrome (pSS) and non-Hodgkin's-MALT type parotid lymphoma before, during and after a standard treatment with cyclophosphamide (CTX) and rituximab (RTX). To identify any discriminatory therapeutic salivary biomarker patient's whole saliva was collected at the baseline, after the fourth infusion of rituximab, and on remission and analysed combining two-dimensional electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry.

Results

Proteomic results obtained from the comparison of salivary samples indicated several qualitative and quantitative modifications in the salivary expression of putative albumin, immunoglobulin J chain, Ig kappa chain C region, alpha-1-antitrypsin, haptoglobin and Ig alpha-1 chain C region.

Conclusion

This study suggests that clinical and functional changes of the salivary glands driven by autoimmune and lymphoproliferative processes might be reflected in patients' whole saliva proteins, shedding new light on the potential usefulness of salivary proteomic analysis in the identification of prognostic and therapeutic biomarkers for patients with pSS and non Hodgkin's lymphomas.

Keywords:
primary Sjögren's Syndrome; B-cell non-Hodgkin's lymphoma; proteomic analysis