Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma
- Equal contributors
1 State Key Laboratory of Oncology in Southern China, Guangzhou 510060, P.R. China
2 Department of Pathology and Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Southern Medical University, Guangzhou 510515, P.R. China
3 Department of Nasopharyngeal Cancer, Cancer Center Sun Yat-sen University, Guangzhou 510060, P.R. China
4 Departments of Experimental Research, Cancer Center Sun Yat-sen University, Guangzhou 510060, P.R. China
5 Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510515, P.R. China
6 First Affiliated Hospital of Jinan University, JiNan 250000, P.R. China
Journal of Translational Medicine 2011, 9:205 doi:10.1186/1479-5876-9-205Published: 1 December 2011
Astrocyte elevated gene-1 (AEG-1) is associated with tumorigenesis and progression in diverse human cancers. The present study was aimed to investigate the clinical and prognostic significance of AEG-1 in salivary gland carcinomas (SGC).
Real-time PCR and western blot analyses were employed to examine AEG-1 expression in two normal salivary gland tissues, eight SGC tissues of various clinical stages, and five pairs of primary SGC and adjacent salivary gland tissues from the same patient. Immunohistochemistry (IHC) was performed to examine AEG-1 protein expression in paraffin-embedded tissues from 141 SGC patients. Statistical analyses was applies to evaluate the diagnostic value and associations of AEG-1 expression with clinical parameters.
AEG-1 expression was evidently up-regulated in SGC tissues compared with that in the normal salivary gland tissues and in matched adjacent salivary gland tissues. AEG-1 protein level was positively correlated with clinical stage (P < 0.001), T classification (P = 0.008), N classification (P = 0.008) and M classifications (P = 0.006). Patients with higher AEG-1 expression had shorter overall survival time, whereas those with lower tumor AEG-1 expression had longer survival time.
Our results suggest that AEG-1 expression is associated with SGC progression and may represent a novel and valuable predictor for prognostic evaluation of SGC patients.