Demonstration of CXCR4 knockdown as determined by mRNA and protein analysis. (A) Mean percent CXCR4 knockdown related to siRNA negative control (NC) as determined by mRNA analysis in Caco-2, HT-29 and SW480 cells. (B) CXCR4 protein expression after CXCR4 siRNA silencing as determined by Western Blot analysis in Caco-2, HT-29 and SW480 cells related to untransfected total cell lysates. Total cell lysates of untransfected cells and one representative example of CXCR4 siRNA transfected cells of each cell line were immunoblotted with antibodies specifically recognizing chemokine receptor CXCR4. Acute leucemia cell line HL60 served as positive control for the detection of CXCR4.
Rubie et al. Journal of Translational Medicine 2011 9:22 doi:10.1186/1479-5876-9-22