Table 2 |
||
|
Applied RNAi control experiments - overview |
||
|
Control experiment |
Type |
Example |
|
|
||
|
Positive control siRNA |
siRNA that is known to provide high knockdown of its target gene |
Hs_MAPK1 siRNA (Qiagen) OrderNo. 1022564 |
|
Negative control siRNA |
a nonsilencing siRNA with no homology to any known mammalian gene |
AllStars Negative control siRNA (Qiagen) OrderNo. 1027280 |
|
Transfection control siRNA |
siRNA that is used to measure the transfection efficiency, e.g. by siRNA |
AllStars Hs Cell Death Control siRNA (Qiagen) OrderNo. 1027298 |
|
Mock transfection control |
control experiment where cells go through the transfection process without addition of siRNA |
|
|
Untransfected cells control |
control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process |
|
|
|
||
|
Rubie et al. Journal of Translational Medicine 2011 9:22 doi:10.1186/1479-5876-9-22 |
||
