Table 2

Applied RNAi control experiments - overview

Control experiment

Type

Example


Positive control siRNA

siRNA that is known to provide high knockdown of its target gene

Hs_MAPK1 siRNA (Qiagen)

OrderNo. 1022564

Negative control siRNA

a nonsilencing siRNA with no homology to any known mammalian gene

AllStars Negative control siRNA (Qiagen)

OrderNo. 1027280

Transfection control siRNA

siRNA that is used to measure the transfection efficiency, e.g. by siRNA

AllStars Hs Cell Death Control siRNA (Qiagen)

OrderNo. 1027298

Mock transfection control

control experiment where cells go through the transfection process without addition of siRNA

Untransfected cells control

control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process


Rubie et al. Journal of Translational Medicine 2011 9:22   doi:10.1186/1479-5876-9-22

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