NBD is effective in inhibiting NF-κB in cardiac muscles from dko mice. Nuclear extracts were prepared from hearts of vehicle (n = 4) or NBD (n = 7) treated dko mice and analyzed by either EMSA (upper panel) or western blot probing for nuclear fraction p65 (bottom panels). Nonspecific band (NS) is shown on the western blot to demonstrate equivalent protein loading.
Delfín et al. Journal of Translational Medicine 2011 9:68 doi:10.1186/1479-5876-9-68